https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15375 Wed 11 Apr 2018 15:55:53 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15374 Wed 11 Apr 2018 15:48:51 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15376 Wed 11 Apr 2018 14:48:05 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:25828 Wed 02 Mar 2022 14:24:33 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:9456 0.05). There was a significant correlation between the greatest percentage fall in forced expiratory volume in 1 s (FEV₁)(r=0.6, P<0.0001), the dose–response slope (r=0.73), cumulative dose (r=0.55) and PD15 (r=0.46) for mannitol and hypertonic saline. The greatest percentage fall in FEV₁to mannitol was less in non-eosinophilic asthma. There was a lower total cell count in mannitol vs. hypertonic-saline-induced sputum. However, sputum eosinophils and neutrophils were not significantly different. Using mannitol, a higher proportion of subjects were classified as having eosinophilic asthma. There were no differences in IL-8, neutrophil elastase or matrix-metalloproteinase 9 concentrations in sputum samples induced with mannitol or hypertonic saline. Conclusion: We conclude that mannitol can be used to induce good-quality sputum, useful for analysis of inflammatory mediators and for predicting the inflammatory phenotype in asthma.]]> Sat 24 Mar 2018 08:41:08 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:2894 Sat 24 Mar 2018 08:32:49 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:12549 Sat 24 Mar 2018 08:16:34 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:18462 3% and > 61% respectively. Peripheral blood monocytes were isolated (n = 61) and sputum macrophages were isolated from a subgroup of patients with asthma (n = 14), using immunomagnetic cell separation. RNA and nuclear proteins were extracted and quantified. Enzyme activity was assessed using fluorescent assays and gene expression of EP300, KAT2B, CREBBP, and HDACs 1, 2 and 3 were measured by qPCR. Results: There was a significant inverse association between blood monocyte HAT and HDAC activity (r = −0.58, P < 0.001). NA was associated with increased blood monocyte HAT enzyme activity (P = 0.02), decreased HDAC activity (P = 0.03), and increased HAT: HDAC ratio (P < 0.01) compared with eosinophilic asthma. There were no differences in gene expression of EP300, KAT2B, CREBBP, or HDACs 1, 2 and 3 in blood monocytes from subjects with asthma or inflammatory phenotypes of asthma. There was no effect of inhaled corticosteroid use, poor asthma control, or asthma severity on HAT/HDAC activities. Sputum macrophages had increased expression of KAT2B in eosinophilic compared with paucigranulocytic asthma. Conclusions and Clinical Relevance: Neutrophilic airway inflammation is associated with increased HAT and reduced HDAC activity in blood monocytes, demonstrating further systemic manifestations relating to the altered inflammatory gene transcription profile of neutrophilic asthma.]]> Sat 24 Mar 2018 07:59:46 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:19229 9/L. The blood eosinophil/lymphocyte ratio (ELR) and eosinophil/neutrophil ratio (ENR) were increased in eosinophilic asthma, and the neutrophil/lymphocyte ratio (NLR) was increased in neutrophilic asthma. Neutrophilic asthma could also be detected by blood neutrophil percentages and NLR, but with less accuracy. Conclusions and Clinical Relevance: Blood eosinophil counts and derived ratios (ELR and ENR) can accurately predict eosinophilic asthma in patients with persistent uncontrolled asthma despite treatment. Blood neutrophil parameters are poor surrogates for the proportion of sputum neutrophils. Blood counts may be a useful aid in the monitoring of uncontrolled asthma.]]> Sat 24 Mar 2018 07:54:52 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:28316 Sat 24 Mar 2018 07:27:05 AEDT ]]>